Results
| PMID | 28923287 |
| Gene Name | LGR5 |
| Condition | Endometriosis |
| Association |
Associated |
| Population size | 51 |
| Population details | 51 (12 healthy women, 39 endometrial women) |
| Sex | Female |
| Other associated phenotypes |
Endometriosis |
|
Fertil Steril. 2017 Nov;108(5):858-867.e2. doi: 10.1016/j.fertnstert.2017.08.018. Vallve-Juanico, Julia| Suarez-Salvador, Elena| Castellvi, Josep| Ballesteros, Agustin| Taylor, Hugh S| Gil-Moreno, Antonio| Santamaria, Xavier Department of Gynecology, IVI Barcelona S.L., Barcelona, Spain; Group of Biomedical Research in Gynecology, Vall Hebron Research Institute (VHIR) and University Hospital, Barcelona, Spain; Universitat Autonoma de Barcelona, Barcelona, Spain.| Departme OBJECTIVE: To characterize leucine-rich repeat containing G protein-coupled receptor 5-positive (LGR5(+)) cells from the endometrium of women with endometriosis. DESIGN: Prospective experimental study. SETTING: University hospital/fertility clinic. PATIENT(S): Twenty-seven women with endometriosis who underwent surgery and 12 healthy egg donors, together comprising 39 endometrial samples. INTERVENTION(S): Obtaining of uterine aspirates by using a Cornier Pipelle. MAIN OUTCOMES MEASURE(S): Immunofluorescence in formalin-fixed paraffin-embedded tissue from mice and healthy and pathologic human endometrium using antibodies against LGR5, E-cadherin, and cytokeratin, and epithelial and stromal LGR5(+) cells isolated from healthy and pathologic human eutopic endometrium by fluorescence-activated cell sorting and transcriptomic characterization by RNA high sequencing. RESULT(S): Immunofluorescence showed that LGR5(+) cells colocalized with epithelial markers in the stroma of the endometrium only in endometriotic patients. The results from RNA high sequencing of LGR5(+) cells from epithelium and stroma did not show any statistically significant differences between them. The LGR5(+) versus LGR5(-) cells in pathologic endometrium showed 394 differentially expressed genes. The LGR5(+) cells in deep-infiltrating endometriosis expressed inflammatory markers not present in the other types of the disease. CONCLUSION(S): Our results revealed the presence of aberrantly located LGR5(+) cells coexpressing epithelial markers in the stromal compartment of women with endometriosis. These cells have a statistically significantly different expression profile in deep-infiltrating endometriosis in comparison with other types of endometriosis, independent of the menstrual cycle phase. Further studies are needed to elucidate their role and influence in reproductive outcomes. Mesh Terms: Biomarkers/analysis| Case-Control Studies| Endometriosis/genetics/*metabolism/pathology| Endometrium/*chemistry/pathology| Female| Fluorescent Antibody Technique| Gene Expression Profiling/methods| High-Throughput Nucleotide Sequencing| Humans| P |
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